Comparison of properties of protein-coding and lincRNA genes. LincRNA genes differ from protein-coding genes with respect to transcript length, number of exons per transcript, number of transcripts per gene, and transcriptional profile. A, Comparison of transcript lengths of coding and noncoding genes. Noncoding genes have shorter transcripts. B, Comparison of the number of transcripts found in coding and noncoding genes. Noncoding genes have less isoforms. C, Comparison of the number of exons found in transcripts of coding and noncoding genes. Transcripts of noncoding genes have a lower number of exons. D, Comparison of log2 (FPKM) values of coding and noncoding genes. FPKM values calculated based on counts produced by featureCounts. Noncoding genes show lower expression levels compared to protein-coding genes. E, Plot presenting distribution of protein-coding and lincRNA loci across 20 soybean chromosomes. LincRNA loci are evenly distributed across chromosomes, whereas protein-coding genes show lower density in centromeric regions. Starting from the outer ring: (1) protein-coding genes, (2) all lincRNA genes, (3) non-TE lincRNA loci, and (4) lincRNA loci with transcripts harboring TEs.