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. 2018 Jan 30;176(3):2251–2276. doi: 10.1104/pp.17.01448

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Figure 4. — Subcellular fluorescence localization of RFP-REIL fusion proteins. The UBQ10::RFP-REIL1 (49) and UBQ10::RFP-REIL2 (109) transformation events of reil1-1 reil2-1 that restored respective single mutant phenotypes (see Fig. 3) were analyzed using seedlings 7 to 10 d after germination at standard temperature (20°C) and after 1 d or more in the cold (10°C). A, Cotyledon epidermis of UBQ10::RFP-REIL1 (49) after in vitro germination and cultivation at 20°C. B, Root tip meristem to transition zone of UBQ10::RFP-REIL1 (49) after in vitro germination and cultivation at 20°C. C, Root tip of UBQ10::RFP-REIL1 (49) after in vitro germination at 20°C and cold shift. D, Cotyledon epidermis of UBQ10::RFP-REIL2 (109) after in vitro germination and cultivation at 20°C. E, Root tip of UBQ10::RFP-REIL2 (109) after in vitro germination and cultivation at 20°C. F, Root tip of UBQ10::RFP-REIL2 (109) after in vitro germination at 20°C and cold shift. Representative analyses include (left to right) false-color image of RFP fluorescence (green), Nomarski differential interference contrast image, and overlay including false-color image of chlorophyll autofluorescence (red). All images are projection stacks of multiple confocal sections. Note the absence of RFP-REIL1 fluorescent signal from vacuolar, chloroplast, and nuclear lumen (A–C). Bars = 25 µm.