Figure 9.

Differential expression of genes that were up-regulated in nonacclimated reil1-1 reil2-1 and changed significantly in 10°C cold-acclimating Col-0. A, DEGs (P < 0.01) in nonacclimated reil1-1 reil2-1 that belonged to GO term GO:0009631, cold acclimation. Note that these three genes also were part of GO:0009409, response to cold. B, Additional DEGs (P < 0.01 or P < 0.05) that belonged to GO term GO:0009409, response to cold. C, Selected genes that belonged to GO:0010286, heat acclimation. Note that DREB2A and DREB2B were increased slightly in the nonacclimated mutant and also part of the long-term Col-0 response to a 10°C shift (see Fig. 8). SKP2B was the only gene of GO:0010286, heat acclimation, that was significantly up-regulated at P < 0.01 in nonacclimated reil1-1 reil2-1. Relative expression values are log₂-FCs (ratios) compared with nonacclimated Col-0 (means ± se). Headers show Arabidopsis gene names and gene codes in parentheses. Significance values of heteroscedastic Student’s t tests comparing nonacclimated mutant and nonacclimated Col-0 are indicated (*, 0.01 < P ≤ 0.05 and **, P ≤ 0.01). Circles in the top right corner of some graphs indicate that the respective genes also were part of the water limitation GO terms, GO:0009414 and GO:0009415.