Figure 1.

Scheme of the high-throughput screen for identifying plant immune stimulants. A quantity of 1-mL aliquots of a 3-d-old parsley cell culture was transferred to individual wells of a 24-well microtiter plate containing a candidate compound for priming (A, B, or C) or the known priming activator SA (positive control). All compounds were dissolved in DMSO (<1%). Thus, DMSO (1%) treatment served as a negative control. Upon incubation for 24 h on a shaker, Pep13 (50 pm) was added to appropriate wells. After shaking for another 24 h, the fluorescence of secreted furanocoumarins was quantified in a microtiter plate reader.