Figure 2.

Role of the –N=C=S group and the oxidation state of sulfur in the side chain. A quantity of 1-mL aliquots of a 3-d-old parsley cell culture in microtiter plates was treated with SFN (A), ERY (B), ERU (C), or SA (A–C, positive control). All compounds were dissolved in DMSO (0.25%). Therefore, treatment with 0.25% DMSO served as an additional control. After 24 h on a shaker, 50 pm Pep13 was added. After another 24 h, furanocoumarin fluorescence in the wells was determined. Data were analyzed by one-way ANOVA followed by posthoc Student’s t test. Different letters denote statistically significant differences with 95% confidence. Data presented are means ± sd (n > 6). RFU, Relative fluorescence units.