Figure 4.

Detection of open chromatin in defense gene promoters/promoter-proximal regions. Arabidopsis plants were left untreated or sprayed with a WP formulation of SFN (450 µm) or BTH (100 µm). WP treatment served as a control for these treatments. After 24 h, we harvested leaves and subjected them to FAIRE. We performed qPCR with primers specific for different sites in the promoter/promoter-proximal region of WRKY6 (A), PDF1.2 (B), PR1 (C), RAB18 (D), and Rubisco (E). Data for every given position of gene were analyzed by one-way ANOVA followed by posthoc Student’s t test. Different letters denote statistically significant differences with 95% confidence. Data presented are means ± sd (n > 6).