Figure 5.

Unprimed or primed defense gene activation in Arabidopsis genotypes treated with SFN or BTH. Wild type (A–D) and ein2-1 (E) plants were left untreated, sprayed with a WP formulation of SFN (450 µm) or BTH (100 µm), or WP only. 24 h later, three leaves of half of the plants for each treatment were challenged by infiltrating flg22 (200 nm; A–D) or left unchallenged (A–E). After 45 min, we extracted RNA from leaves and subjected it to RT-qPCR analysis using gene-specific primers (Supplemental Table S1). Data were analyzed by one-way ANOVA followed by posthoc Student’s t test. Different letters denote statistically significant differences with 95% confidence. Data presented are means ± sd (n > 6).