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. 2018 Mar 7;13(3):e0193109. doi: 10.1371/journal.pone.0193109

Table 1. Ingredients, chemical analysis, and calculated energy for the primary diets.

Primary diet treatment
Low roughage High roughage
Ingredient, %
Corn grain (flaked) 62.3
Dried distillers grains with solubles (corn based) 15.0
Tallow 2.5
Molasses (cane) 6.0
Alfalfa hay (early bloom) 6.0
Wheat hay 6.0
Sudan grass hay 100
Limestone 1.12
Urea 0.57
Magnesium oxide 0.14
Cobalt sulphate (21% Co) 0.00004
Copper sulphate (25.4% Cu) 0.002
Manganese sulphate 0.0095
Potassium iodide (68% I) 0.000045
Salt 0.3
Rumensin 90a (41.2 g/kg) 0.02
Zinc Oxide (78% Zn) 0.0027
Chemical analysis
Dry matter (DM), % 83.6 88.8
CP, % DM 12.6 8
NDF, % DM 17.9 60.3
ADF, % DM 8.6 41.9
Calculated energy
TDN, % DM 84.7 56.4
NEm, MJ/kg 8.64 4.97
NEg,MJ/kg 5.89 2.57

The primary diet treatments were a total mixed ration with 12% forage (low roughage) or 100% Sudan grass hay (high roughage). Ingredient composition is listed on an as-fed basis. Chemical analysis and energy calculations were performed by Cumberland Valley Analytical Services, Hagerstown, MD, U.S.A. using the formulas of Weiss [20] and NRC [21].

aElanco Animal Health, Greenfield, IN, U.S.A.