Table 2. Total number of proteins and peptides in spectral library.
| GBM patients | Number of specimens | Number of identified | |
|---|---|---|---|
| Proteins | Peptides | ||
| Plasma | 14 | 216 | 1,689 |
| Plasma (IEF) | 1 | 282 | 2,642 |
| Cyst fluid | 3 | 266 | 1,074 |
| Cyst fluid (IEF) | 1 | 811 | 4,331 |
| Tumor tissue | |||
| Whole tissue lysate | 14 | 761 | 2,236 |
| Cytosol fraction | 14 | 684 | 2,521 |
| Microsome fraction | 14 | 1,280 | 4,632 |
| Plasma membrane fraction | 13 | 2,590 | 14,523 |
| Total number in spectral library | 2,930 | 20,066 | |
Plasma obtained from subject P14 and cyst fluid obtained from subject P1 were divided into 12 fractions with a 3–10 linear pH range by preparative isoelectric focusing (IEF). Only 13 plasma membrane fractions of glioblastoma (GBM) patients could be obtained, because the tissue volume of subject P13 was too small for preparing plasma membrane fraction. Modified peptides were not included in the number of identified peptides. Only proteins registered in SWISS-PROT were included in the number of identified proteins. All isoforms of a protein were counted as one.