Table 3. Summary of the differentially expressed proteins identified in plasma analyzed by SWATH-MS analysis.

Protein	Peptide	AUC	p value	Effect size
Up-regulated proteins
Complement component C9	LSPIYNLVPVK	0.871	7.35×10−4	1.59
	VVEESELAR*	0.867	2.33×10−3	1.40
Leucine-rich alpha-2-glycoprotein	ALGHLDLSGNR*	0.867	1.61×10−3	1.45
	DLLLPQPDLR	0.867	3.74×10−3	1.32
C-reactive protein	ESDTSYVSLK*	0.793	6.96×10−2	0.761
Apolipoprotein B-100	LATALSLSNK*	0.790	9.69×10−3	1.13
Alpha-1-antichymotrypsin	DSLEFR	0.771	2.40×10−2	0.977
	ITLLSALVETR	0.733	2.60×10−2	0.960
	NLAVSQVVHK*	0.729	3.28×10−2	0.910
Down-regulated proteins
Gelsolin	TGAQELLR	0.962	3.16×10−5	2.03
	HVVPNEVVVQR*	0.876	2.37×10−4	1.57
Ig alpha-1 chain C region	DASGVTFTWTPSSGK*	0.862	8.39×10−4	1.40
Apolipoprotein A-IV	LTPYADEFK	0.814	2.82×10−3	1.22
	IDQNVEELK	0.800	2.13×10−3	1.32
	LEPYADQLR*	0.790	2.62×10−3	1.23

Receiver operating characteristics analysis and Student’s t-test were performed for the peak areas obtained by SWATH mass spectrometry (SWATH-MS) analysis of healthy plasma (n = 15) and glioblastoma plasma (n = 14). Peptides shown in bold with an asterisk (*) were selected as the most reliable peptide representing each protein for quantitative targeted absolute proteomics analysis based on the peak shape of chromatograms. AUC, area under the curve