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. 2018 Mar 7;13(3):e0193964. doi: 10.1371/journal.pone.0193964

Fig 1. In ovo delivery of CpG DNA increases KUL01+ and CD4+ cell numbers in lungs pre-hatch.

Fig 1

SPF ED18 eggs were injected with CpG DNA (n = 9), non-CpG DNA (n = 4) or PBS (n = 6). At ED19, lungs were collected and immunofluorescent assay was performed for KUL01+ and CD4+ cells. The quantitative data for KUL01+ (a) and CD4+ cells (b) are shown. The one-way ANOVA test with Bonferroni's post test for selected comparison was performed to identify group differences and the differences were considered significant at P< 0.05. The bars represent mean ± SEM.