Fig 5. ILTV genome loads and KUL01+, IgM+ B, CD4+ and CD8α+ cell numbers in lungs of in ovo CpG DNA and non-CpG DNA treated chickens following ILTV infection post-hatch.

The chickens were delivered with either in ovo CpG DNA or non-CpG DNA and infected with ILTV intra-tracheally at day 1 post-hatch. The chickens were observed for end points. The lungs were collected for determination of ILTV genome loads and cellular responses from chickens that reached humane endpoint at 6 days post-infection and experimental end point at 12 days post-infection. The ILTV genome loads are given separately for 6 and 12 days post-infection (a). The quantitative data of the immunofluorescent assays done for IgM+ B cells (b), KUL01+ (c), CD4+ cells (d) and CD8α+ cells (e) are shown separately for 6 and 12 days post-infection. Statistical analysis was precluded due to low numbers of animals per group per time point. The bars represent mean ± SEM.