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. 2018 Mar 7;13(3):e0194089. doi: 10.1371/journal.pone.0194089

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© 2018 Qian et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Shows immunostaining of CHRNA4 (A; α4) and CHRNA5 (B; α5) in HBO cells using 20x and 40x objectives (total 200x and 400x magnification). The panels show merged confocal images of DAPI (blue) and secondary antibody fluorescence (green). The negative control (NC) without primary antibody is also shown. The horizontal red lines represent 10 μm.