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(A, B, C) Single cell real-time RT-PCR technique was used to perform expression analysis of CHRN subunits using the Single Cell-to-CTTM Kit in 32 individual HBO cells. The final real-time PCR products were separated by electrophoresis on a 2% agarose gel containing 1 μg/ml ethidium bromide. In (A) T2R38 was not detected in cell number 1–11.
