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. 2018 Mar 7;13(3):e0194089. doi: 10.1371/journal.pone.0194089

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© 2018 Qian et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 13 — HBO cells were treated with Nic (0.25–1.0 μM), ETOH (50 mM), and 0.5 μM Nic + 50 mM ETOH for 24h. Western blots were developed using specific AChRα5 or AChRα6 antibodies (A) and analyzed (B and C). The fold change in protein expression was calculated with respect to β-actin. The values are means of triplicate measurements.