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. 2018 Mar 7;13(3):e0194089. doi: 10.1371/journal.pone.0194089

Fig 14. Effect of Nic exposure on the CHRN subunit mRNA expression level in HBO cells.

Fig 14

(A) After 24h Nic treatment. Relative to control, at 0.25 μM Nic the *p values for CHRNA3, CHRNA5, CHRNA6, CHRNB2, and CHRNB4 mRNAs were 0.0111, 0.0306, 0.0017, 0.0239, and 0.0173, respectively. Relative to control, at 0.50 μM Nic the *p values for CHRNA3, CHRNA5, CHRNA6, CHRNB2, and CHRNB4 mRNAs were 0.0022, 0.0473, 0.0014, 0.0049, and 0.0141, respectively. Relative to control, at 1.0 μM Nic the *p values for CHRNA3, CHRNA5, CHRNA6, CHRNB2, and CHRNB4 mRNAs were 0.0194, 0.0055, 0.0013, 0.0236, and 0.0003, respectively. Relative to 0.25 μM Nic, at 0.50 μM Nic the *p values for CHRNA6 and CHRNB4 were 0.0039 and 0.0024. Relative to 0.50 μM Nic at 1.0 μM Nic the *p values for CHRNA6, CHRNB2, and CHRNB4 were 0.0192, 0.0144, and 0.0046, respectively. (B) After 24h ETOH treatment. Relative to control, at 50 mM ETOH the *p values for CHRNA3, CHRNA5, CHRNA6, and CHRNB2 mRNAs were 0.0048, 0.0207, 0.0020, and 0.0169, respectively. Relative to 50 mM ETOH, at 50 mM ETOH + 0.5 μM Nic the *p values for CHRNA3 and CHRNA6 mRNAs were 0.0049 and 0.0024. (C) After 4 days treatment. Relative to control, at 0.25 μM Nic the *p values for chrna6 and CHRNA7 mRNAs were 0.0003 and 0.024, respectively. After 4 days treatment, relative to control, at 0.50 μM Nic the *p values for CHRNA5, CHRNA6, CHRNA7, and CHRNB4 were 0.0402, 0.0001, 0.0049, and 0.0001, respectively. Relative to control, at 1.0 μM Nic, the *p values for CHRNA5, CHRNA6, CHRNA7, and CHRNB4 were 0.0009, 0.0005, 0.0001, and 0.0001, respectively. The values represent mean ± SEM of triplicate measurements.