Table 1. RT-PCR primers for CHRN subunits.

Gene Product	Sequence	NCBI Reference Sequence
CHRNA3 F	GGTGGACGACAAGACCAAAG	NM_000743.4
CHRNA3 R	GGGAAGTAGGTCACGTCGATT
CHRNA4 F	GGAGGGCGTCCAGTACATTG	NM_000744.6
CHRNA4 R	GAAGATGCGGTCGATGACCA
CHRNA5 F	AAAGATGGGTTCGTCCTGTGG	NM_000745.3
CHRNA5 R	CAAACAAAACGATGTCTGGTGTC
CHRNA6 F	TGAGACTCTTCGCGTTCCTG	NM_001199279.1
CHRNA6 R	ATTTCAGCTTTGTCATACGTCCA
CHRNB2 F	CAATGCTGACGGCATGTACGA	NM_000748.2
CHRNB2 R	CACGAACGGAACTTCATGGTG
CHRNB4 F	AACCCGTTACAATAACCTGATCC	NM_000750.4
CHRNB4 R	ATTCACGCTGATAAGCTGGGC

F = forward; R = reverse

Except for CHRNA6, all the primers for other CHRNs were same as described by Hochheimer et al. [6]