Table 2. RT-PCR primers for GPCRs and signaling components.
Gene Product | Sequence | NCBI Reference Sequence |
---|---|---|
PLCβ2 F | CACCCCAGGGGCTATAAGAG | NM_004573.2 |
PLCβ2 R | GGACAGGGTTGAGCAGAGAC | |
T1R1 F | CGGAGTCTTCTCCTGACTTCA | NM_138697.3 |
T1R1 R | CCGTGGAGTTGTTTATCTCCTC | |
T1R3 F | CCGCCTACTGCAACTACACG | NM_152228.2 |
T1R3 R | CTAGCACCGTAGCTGACCTG | |
T2R38 F | TCCCTGGGAAGGCACATGAG | NM_176817.4 |
T2R38 R | CAGCACAGTGTCCGGGAATC | |
TRPM5 F | GTGACCTGGAGGAGGTGATG | NM_014555.3 |
TRPM5 R | AGCAGGCTCTTGCGTGAC |
F = forward; R = reverse