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. 2017 Oct 5;2(19):e95367. doi: 10.1172/jci.insight.95367

Figure 7. CMV-specific mc IgGs as determined by the MIAA assay.

Figure 7

(A) Results of the multiplex infectious-antigen array (MIAA) assay for the 2 patients found to have a CMV-specific monoclonal (mc) IgG. For each patient, the results obtained for the CMV lysate and a mix of 5 CMV Ags (see Methods) with the patient’s serum (S) and purified mc IgG are shown. (B) Detail of results obtained for patient P84: serum P84 contained IgG that recognized CMV, EBV nuclear antigen-1 (EBNA-1), EBV viral capsid antigen (VCA), H. pylori, herpes simplex virus-1 (HSV-1), HSV-2, and varicella zoster virus (VZV) ORF-26, whereas the purified mc IgG recognized only the CMV lysate. (C) Detail of results obtained for patient P112: serum P112 contained IgG that recognized B. burgdorferi, CMV, EBNA-1, EBV VCA, H. pylori, HSV-1, HSV-2, T. gondii, and VZV, whereas the purified mc IgG recognized only the CMV Ag mix. The fluorescence values shown for each pathogen, Ag, or lysate were obtained after subtraction of the threshold of specific positivity of the pathogen, Ag, or lysate (1,000 for CMV). Dots may be superimposed; horizontal bars represent the means of results obtained for a pathogen, Ag, or lysate. Experiments were performed at least twice.