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. 2017 Oct 5;2(19):e94275. doi: 10.1172/jci.insight.94275

Figure 4. DOCK8 is dispensable for Tregs homing.

Figure 4

(A) RFP+ (Foxp3+) Tregs (CD45+CD4+ gated) were analyzed in spleen, LN, and Lungs by flow cytometry at day 2 after tamoxifen injections. Results described in this figure are representative of 2 independent experiments with minimum of 2 mice per group. (B) RFP+ (Foxp3+) Tregs (CD45+CD4+ gated) were analyzed in spleen, LNs, and lungs of Foxp3CreERT2 R26RFP, Foxp3CreERT2DOCK8fl/fl R26RFP, and Foxp3CreERT2S1P1fl/fl R26RFP by flow cytometry at day 10 after tamoxifen injections. Data represents 2 independent experiments with 2–3 mice per group. (C and D) CD4+Foxp3+ Tregs were analyzed in the lungs of Foxp3CreERT2Cdc42fl/fl and Foxp3CreERT2DOCK8fl/fl mice by flow cytometry at day 10 after tamoxifen injections. FACS plot (C) and scatter plot (D) show the frequency and total Tregs in the lung of control and tamoxifen-injected Foxp3CreERT2DOCK8fl/fl and Foxp3CreERT2Cdc42fl/fl mice. Data represents 3 independent experiments with minimum of 3 mice per group. The data shown are the mean ± SD. Statistics were performed with Prism software by using 1-way ANOVA. **P < 0.01, ***P < 0.001.