Fig. 4.

BMPR2 mutation and WD have varied results on small pulmonary vessels. (a) Small pulmonary vessel density was not altered by diet or genotype. Mean vessels per 10× high power field is given (averaged over ten fields, n = 3–5 mice per bar). (b) Pulmonary vessels in mice with BMPR2 mutation showed increased medial wall thickness, but this did not vary based on diet exposure (two-way ANOVA P < 0.01 for effect of genotype, P = NS for effect of diabetogenic exposure). The mean medial wall thickness from all vessels on 15 high powered fields is given for n = 4 mice. (c) WD and mutation each significantly increased lung ROS (P < 0.01 for WD, P < 0.05 for BMPR2 mutation, n = 10–12 per bar). ROS given as fold change compared with control genotype, SD group. Comparison with SD-fed group of same genotype, *P < 0.05.