Fig. 8.

Insulin-induced translocation of glucose transporter type 4 is reduced by BMPR2 mutation. GLUT4 immunolocalization (green) was demonstrated at various time points of insulin exposure in endothelial cells co-stained with DAPI (blue) and actin (red). The proportion of cells with GLUT4 predominantly located in a perinuclear region versus diffusely throughout the cytoplasm was represented graphically. *P < 0.05 compared to basal condition (zero minutes); ^‡P < 0.05 compared to cells without mutation. Statistical comparison is by chi-square test.