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. Author manuscript; available in PMC: 2018 Mar 7.
Published in final edited form as: ACS Comb Sci. 2017 Jul 13;19(8):533–543. doi: 10.1021/acscombsci.7b00080

Figure 6.

Figure 6

NF-κB induction assay and MTT toxicity in THP-1 cells. The viability (blue boxes) measured by MTT assay in THP-1 cells relative to DMSO (blue dotted line) and NF-κB percent activation for synthesized pyrimidoindole library compounds in the presence of LPS calculated relative to LPS alone (black dotted line) values at both 5 h (black boxes) and 20 h (red boxes). The values were statistically computed from two and four independent experiments for MTT cell viability and NF-κB activation assays, respectively in THP-1 cells. The C2–S-methylene substituent on the pyrimidoindole for the synthesized compounds is shown in the individual blocks on the left. Relative viability was calculated as a percentage of OD at 630 nm compared to DMSO. The OD at 630 nm for DMSO was 0.92 ± 0.1 (mean ± SD). Percent activation (%act) was calculated as a response ratio relative to LPS. The response ratio for LPS was 8.00 ± 1.2 and 2.58 ± 0.48 at 5 h and 20 h (mean ± SD), respectively.