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. 2018 Mar 1;13:1241–1256. doi: 10.2147/IJN.S158290

Figure 4.

Figure 4

(A) Cytotoxicity of NPs detected by MTT assays. SW480 cells were treated with blank control, MSNs@PDA, MSNs@PDA-Apt, MSNs-anti-miR-155@PDA, and MSNs-anti-miR-155@PDA-Apt for 12, 24, and 48 hours. Data are expressed as mean ± SEM (*P<0.05, **P<0.01). (B) Cell proliferation evaluation by BrdU incorporation assay. SW480 cells were treated for 12 hours (green, BrdU; blue, DAPI), scale bar =10 μm. (C) Clone formation evaluation of MSNs-anti-miR-155@PDA-Apt.

Abbreviations: OD, optical density; NC, negative control; MSNs, mesoporous silica nanoparticles; PDA, polymerized dopamine; Apt, aptamer; BrdU, 5′-bromo-2′-deoxyuridine; DAPI, 4′,6-diamidino-2-phenylindole; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; SEM, standard error of mean; miR-155, MicroRNA-155.