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. 2018 Feb 20;22(8):2066–2079. doi: 10.1016/j.celrep.2018.01.089

Figure 5.

Figure 5

Lysosomal Activity Impairment in PD-OPA1 NPCs

(A–H) Representative images of NPCs stained with LAMP1 (A–D) and LysoTracker Red (E–H).

(I and J) Quantification of LAMP1 (I) and LysoTracker (J) stainings show a loss of lysosomal acidic pH in PD-OPA1 NPCs. Quantification was performed by analyzing the number of dots normalized on the cell surfaces.

(K and N) Immunoblot analysis (K) and relative quantification (N) for LAMP1 indicates a comparable lysosomal content in PD-OPA1 and ND NPCs.

(L and O) Immunoblot analysis (L) and quantification of intensity (O) of p62 protein levels after normalization to the housekeeping protein.

(M and P) Immunoblot analysis (M) and relative quantification (P) for cathepsin D (CTSD) reveals a reduction of intensity of CTSD in PD-OPA1 NPCs.

Data are mean ± SEM, n = 3–4 independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Statistical analysis was performed using one-way ANOVA followed by Tukey post-test. Scale bars, 100 μm (A–D), 50 μm (E–H).