Figure 7.

Inhibition of Necroptosis Attenuates Nigrostriatal Degeneration Induced by MPTP Treatment

(A–C) Representative pictures of TH⁺ fibers in the striatum of animals used for Nec-1 treatment.

(I–K) Representative pictures of TH⁺ fibers in the striatum of animals used for Nec-1s treatment.

(D and L) Quantification of TH⁺ fiber density calculated as the mean of the level of greys (8-bit) showed a reduction in MPTP-treated mice compared with saline-injected mice and a partial restoration in both Nec-1 (D) and Nec-1s (L) groups.

(E–G) Representative pictures of TH⁺ neurons in SN animals used for Nec-1 treatment.

(M–O) Representative pictures of TH⁺ neurons in SN animals used for Nec-1s treatment.

(H and P) Stereological quantification highlights a decrease in the total number of TH⁺ neurons in animals receiving MPTP. The neuronal loss was prevented by Nec-1 (H) and Nec-1s (P) treatment.

Values are means ± SEM; n = 5–8 animals. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Statistical analysis was performed using one-way ANOVA followed by Tukey post-test. Scale bar, 2 mm (A–C and J–L), 400 μm (E–G, and M–O).