Fig 9. Activation of NF-κB in HEK-Blue hTLR4 cells by heat-killed wild type K96243 and mutant containing unmarked deletions of wbiD, wbiA, and oacA.

HEK-Blue hTLR4 cells were stimulated with heat-killed bacteria at 10⁶ or 10⁷ CFU/ml at 37°C in 5% CO₂ for 24 h. The positive control was E. coli ATCC 25922. NF-κB activation was determined in the supernatant by a SEAP reporter assay. Mean ± SD of two independent experiments were illustrated.