Fig 10. Activation of NF-κB in HEK-Blue hTLR4 cells by heat-killed wild type 4095A and mutant containing unmarked deletions of wcbB.

The HEK-Blue hTLR4 cells were stimulated with heat-killed bacteria at 10⁶ or 10⁷ CFU/ml at 37°C in 5% CO₂ for 24 h. NF-κB activation was determined in the supernatant by a SEAP reporter assay. Mean ± SD of two independent experiments were illustrated.