(A) Q-PCR analysis of IL-1β mRNA expression. Compared to control mice, the inflammatory cytokine IL-1β was upregulated 32-fold in olfactory mucosa derived from Dox treated IOI mice. IOI mice were treated with Dox for 3 weeks (n = 3). *P = 0.0001.
(B) Quantification of Caspase-3+ apoptosis in olfactory sensory neurons (OSNs).
(C) Representative confocal images of caspase-3 immunostaining. IOI mice were treated with Dox for 3 weeks (n = 3). Scale bar, 20 μm.