Fig. 2.

Regulation of arginine metabolic pathway by myeloid P2Y2 receptor during acute inflammation. a WT-tp and P2Y2^−/−-tp mice were dosed i.p. with 2 mg/kg LPS for 24 h, and peritoneal lavage was performed. mRNA levels of nitric oxide synthase (Nos2), arginase 1 (Arg1), interleukin 6 (Il6), tumor necrosis factor alpha (Tnfα), interleukin 1-beta (Il1β), and interleukin 10 (Il10) were determined by qRT-PCR. Data is normalized to β2-microglobulin mRNA and expressed as mean ± SEM, *p < 0.01 by unpaired, two-tailed Student’s t test. b Bone marrow-derived macrophages were obtained from WT and P2Y2 receptor null mice and treated with 1 μg/mL LPS for 6 h. Experiment was performed in quadruplicate. Data is normalized to β2-microglobulin mRNA and expressed as fold change from untreated control, mean ± SEM, *p = 0.02 by unpaired, two-tailed Student’s t test with Welch’s correction