Fig. 1.

P2Y₁₂ receptor in the DRG is upregulated in gp120+ddC-treated rats. a Expression of P2Y₁₂ receptor mRNA in the DRG was measured by RT-qPCR. Expression in the gp120+ddC group was higher than in the sham group (p < 0.01). In gp120+ddC rats treated with P2Y₁₂ receptor shRNA, expression was significantly lower than in the gp120+ddC rats (p < 0.01). The experiment was performed three times (n = 8 per group). Data are presented as the means ± SE. **p < 0.01 compared to the sham group; ##p < 0.01 compared to the gp120+ddC-treated group. b Expression of P2Y₁₂ receptor protein in the DRG was assessed by Western blotting. Protein expression in the gp120+ddC-treated group was increased compared to the sham group (p < 0.01). In gp120+ddC rats treated with P2Y₁₂ receptor shRNA, expression was significantly lower than in the gp120+ddC rats (p < 0.01). Bar graphs show the ratio of the P2Y₁₂ receptor protein level to β-actin level in each group. Data are displayed as the means ± SE. **p < 0.01 compared to the sham group; ##p < 0.01 compared to the gp120+ddC group