Fig. 8.
Attenuation of ExoHep binding to hepatocytes by siRNAs to integrin αv or integrin β1. a qRT-PCR for integrin αv mRNA in control or si-integrin αv-transfected AML12 cells (left panel) (inset: Western blot using anti-αvβ3 or anti-β-actin) or qRT-PCR for integrin β1 mRNA in control or si-integrin β1-transfected AML 12 cells (right panel) (inset: Western blot using anti-integrin α5β1 or anti-β-actin). b Effect of knockdown of integrin αv (left panel) or integrin β1 (right panel) in hepatocytes on the ability of the cells to bind PKH26-ExoHep (8 μg/ml) over 24 h, as determined by spectrophotometric quantification of fluorescence in cell lysates. “Ctrl” is cells treated with a scrambled siRNA sequence. (*p < 0.001 vs. ctrl., student’s t-test)