Fig. 2.

Endoglin heterozygote mice are resistant to bleomycin-induced skin fibrosis. a Hematoxylin and Eosin (H & E) staining of skin sections from endoglin heterozygote mice and wild-type littermates injected with bleomycin or PBS for 28 days. Vertical green line indicates a representative measurement of dermal thickness which was determined as the distance from the superficial aspect of the hypodermis to the epidermal–dermal junction at the edge of the wound. Scale bar = 500 μM. b Quantitative analysis indicates that endoglin heterozygote mice display a resistance to bleomycin-induced skin fibrosis (dermal thickening) as compared to wild-type littermates. *P < 0.05; **P < 0.005. c Picrosirius red staining of skin sections from endoglin heterozygote mice and wild-type littermates injected with bleomycin or PBS for 28 days. The bleomycin-induced increase in birefringence was markedly reduced in endoglin heterozygote mice as compared to their WT littermates