Time-lapsing imaging of organoid growth and response to drug therapies. A) Growth percentage and images of organoids following 1.25–10 μM 5-FU; i) Growth curves of LM7727, ii) Growth curves of LM8516, iii) Growth curves of LM8684 and iv) Representative images of LM8684 following 5-FU treatment. B) Growth percentage and images of organoids following 1.25–5 μM Oxaliplatin; i) Growth curves of LM7727, ii) Growth curves of LM8516, iii) Growth curves of LM8684 and iv) Representative images of LM8684 following Oxaliplatin treatment. C) Growth percentage and images of organoids following 2.5–10 μM Irinotecan; i) Growth curves of LM7727, ii) Growth curves of LM8516, iii) Growth curves of LM8684 and iv) Representative images of LM8684 following Irinotecan treatment. All images were taken at 4 × magnification. *Indicates statistically significant, p ≤ 0.05.