Table 1.
Hindbrain microdissection for western blot analysis of Tph2 protein expression in Experiment 2.
| Brain region (DR subdivision) | Distance from bregma | Inner diameter of microdissection needle | Total number of microdissections collected (pooled) |
|---|---|---|---|
| DRD | −7.50 mm | 410 μm | 3 |
| −7.80 mm | 410 μm | ||
| −8.10 mm | 410 μm | ||
| DRV | −7.50 mm | 410 μm | 3 |
| −7.80 mm | 410 μm | ||
| −8.10 mm | 410 μm | ||
| DRVL/VLPAG (left and right side) | −7.80 mm | 410 μm (x 2) | 4 |
| −8.10 mm | 410 μm (x 2) | ||
| DRC | −8.40 mm | 690 μm | 2 |
| −8.70 mm | 690 μm | ||
| DRI | −8.40 mm | 410 μm | 2 |
| −8.70 mm | 410 μm |
For each brain region (DR subdivision), the total number of microdissections (2–4) were pooled to allow for proper signal detection. Abbreviations: DRC, dorsal raphe nucleus, caudal part; DRD, dorsal raphe nucleus, dorsal part; DRI, dorsal raphe nucleus, interfascicular part; DRV, dorsal raphe nucleus, ventral part; DRVL, dorsal raphe nucleus, ventrolateral part; VLPAG, ventrolateral periaqueductal gray.