Fig. 1.

FCPR16 suppressed MPP⁺-induced cell viability lose in SH-SY5Y cells. (A)The chemical structure of FCPR16. (B) SH-SY5Y cells were treated with various concentration (3.1–50 μM) of FCPR16 or 0.1% DMSO (vehicle control) for 1 h and then followed by incubation with MPP⁺ (500 μM) for 48 h and cell viability was measured using the CCK-8 assay. (C) Cells were pre-treated with FCPR16 (25 μM) or 0.1% DMSO (vehicle control) for 1 h and then incubated with or without 500 μM MPP⁺ for 48 h and the activity of lactate dehydrogenase (LDH) was detected by LDH cytotoxicity assay kit. (D) Cells were exposed to MPP⁺ (500 μM) in the absence and presence of FCPR16 for 48 h, apoptotic cells were determined by Hoechst assay, Bar = 200 µm. (E) Quantification of apoptotic cells, the number of apoptotic nuclei with condensed chromatin was counted from the photomicrographs and presented as a percentage of the total number of nuclei. Results are shown as the mean ± SD and represent three independent experiments. n = 3. ^##P < 0.01, ^###P < 0.001, compared with control group ^*P < 0.05, ^**P < 0.01, ^***P<0.001, compared with MPP⁺ treated group.