Fig. 4.

Effects of FCPR16 on intracellular levels of cAMP and Epac-2. (A) SH-SY5Y cells were incubated with media alone or indicated concentration of FCPR16 for 160 min. Lysates of the cells were assayed using cAMP ELISA kit. Results are shown as the mean ± SD and represent three independent experiments. n = 3. ^*P < 0.05, ^***P < 0.001 compared with control group. (B) Cells were pre-incubated with by FCPR16 (25 µM) for 1 h, and then cells were treated with MPP⁺ (500 µM) for 4 h. Levels of Epac-2 were measured in cellular extracts using Western blot. Data shown are mean ± SD, ^###P < 0.001 compared with control group, *P < 0.05 compared with MPP⁺-treated group.