Fig. 5.

FCPR16 stimulated the phosphorylation of Akt and CREB. (A) SH-SY5Y cells were treated with 25 μM FCPR16 for various times, (B) SH-SY5Y cells were treated with FCPR16 in different concentrations. Expression of phosphated Akt and CREB were analyzed by immunoblotting. (C-F) Relative levels of p-Akt versus total Akt and p-CREB versus total CREB in each sample determined by densitometry of the blots. Densitometric analysis of the immunoblot was expressed as a percentage of control. Results are shown as the mean ± SD and represent three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared with control.