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. 2018 Feb 14;16:47–58. doi: 10.1016/j.redox.2018.02.008

Fig. 6.

Fig. 6

KRX-0401 and H-89 blocked the phosphorylation of Akt and CREB induced by FCPR16, respectively, in SH-SY5Y cells. (A) Following treatment with KRX-0401 for 1 h, SH-SY5Y cells were exposed to MPP+ in the presence of FCPR16 (25 μM) for 4 h, and the phosphorylation of Akt was determined by Western blot. (B) Relative levels of p-Akt versus total Akt in each sample determined by densitometry of the blots. (C) Following treatment with H-89 for 1 h, SH-SY5Y cells were exposed to MPP+ in the presence of FCPR16 (25 μM) for 4 h, and the phosphorylation of CREB was determined by Western blot. (D) The phosphorylation of CREB was analyzed by immunoblotting. Densitometric analysis of the immunoblot was expressed as a percentage of control. Results are shown as the mean ± SD and represent three independent experiments. ##P < 0.01, ###P<0.001, compared with control. *P < 0.05 compared with MPP+, $P < 0.05, compared with (FCPR16 + MPP+) treated group.