Fig. 6.

Effects of MitoQ on NLRP3, TRX and TXNIP expression and intracellular ROS production in HK-2 cells exposed to different concentrations of D-glucose. A: Representations of intracellular ROS levels (A, upper panel), MMP (A, middle panel) and mitochondrial ROS levels (A, bottom panel) in HK-2 cells subjected to HG treatment with or without MitoQ pretreatment. B-F: Quantification of intracellular ROS production as measured with DCFDA staining (B), MMP as measured with TMRE staining (C), mitochondrial ROS production as measured with MitoSox Red staining (D), cellular H₂O₂ production (E) and Ca²⁺ loading for MPTP opening (F) in HK-2 cells treated with or without different concentrations of MitoQ. G-J: WB and densitometric analysis of NLRP3, TRX, TXNIP, Caspase-1, IL-1β, Collagen I and FN expression in HK-2 cells exposed to different concentrations of D-glucose and treated with or without MitoQ. Values are the mean ± SE, *P < 0.05, vs control group; ^#P < 0.05 vs HG group. n = 3.