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. 2018 Feb 15;16:32–46. doi: 10.1016/j.redox.2018.02.013

Fig. 8.

Fig. 8

Effects of MitoQ, MSU, TRX siRNA and TXNIP siRNA on TXNIP/NLRP3/IL-1β axis and pro-fibrotic protein expression and apoptosis in HK-2 cells cultured under HG conditions. A: IF showing FN (Aa-c) and Collagen I (Ad-f) expression in HK-2 cells subjected to HG treatment following MitoQ treatment. B: WB analysis of Caspase-1, cleaved IL-1β and IL-18 expression in HK-2 cells cultured under HG conditions and treated with different concentrations of MitoQ (50,100, 150 nM). C-H: ELISA of Caspase-1 (C), IL-1β (D), IL-18 (E), TGF-β (F) activity; TGF-β expression (G); and relative apoptosis (H) in HK-2 cells subjected to HG conditions and treated with different concentrations of MitoQ (50, 100, 150 nM). I: Luminex analysis of IL-1β, IL-18, FN and Collagen I expression in HK-2 cells subjected to an HG environment and treated with MitoQ, MSU or TXNIP siRNA. J, K: WB and densitometric analysis of NLRP3, IL-1β, IL-18 and FN expression in HK-2 cells exposed to an HG environment and pretreated with MitoQ, TRX siRNA or TXNIP siRNA. Values are the mean ± SE; *P < 0.05 vs control group; #P < 0.05 vs HG group. n = 3.