Figure 5.

Soluble Tyro3 (sTyro3) promoted osteoclast differentiation in rheumatoid arthritis (RA) patients. Freshly isolated peripheral blood mononuclear cells (PBMCs) were cultured with or without rhTyro3 Fc (500 ng/ml) under the stimulation of recombinant human macrophage colony‐stimulating factor (rhM‐CSF) (30 ng/ml) and recombinant human receptor activator of nuclear factor kappa‐Β ligand (rhRANKL) (50 ng/ml). Medium was changed every 3 days. Then, cells were subjected to tartrate‐resistant acid phosphatase (TRAP) staining on day 17. One representative experiment of six (a) and the statistical results were shown (b). The analysis was performed by Wilcoxon's matched‐pairs signed‐rank test, *P < 0·05. [Colour figure can be viewed at wileyonlinelibrary.com]