Fig. 4.

RhoA/ROCK inhibition promotes NSC migration on CS. a, b NSC migrate significantly longer distances in the presence of 10 μM of Y27632 on laminin + CS, 18 h after plating (***p = 0.0004). Scale bar at 200 μm. c, d Inhibition of ROCK increases NSC area 1 and 3 h after plating on laminin + CS (***p = 0.0001). At least 30 cells were measured for each condition in three independent experiments. Eighteen hours after plating, NSC do not form cell clusters on CS in the presence of Y27632, as it was observed without ROCK inhibition. e, f TIRF analyses revealed that adhesions were significantly smaller on cells plated on laminin + CS in the presence of ROCK inhibitor (**p = 0.0188). Number of adhesions analyzed: CS = 71; CS + Y27632 = 30. Scale bar at 6 μm