Table 6.
Influence of the CUMS on the parameters of oxidative stress (TAC, GPx, SOD, MDA) within the whole brain and its structures (cortex, hippocampus, and cerebellum) in rats after 3 days of saline or nicotine (0.175 mg/kg, i.p.) conditioning
| Oxidative stress parameter | Group | Control | CUMS | ||
|---|---|---|---|---|---|
| Conditioning | Saline | Nicotine | Saline | Nicotine | |
| TAC | Brain | 459.60 ± 4.86 | 428.20 ± 8.30*** | 428.50 ± 14.66*** | 402.60 ± 9.83###, ^^^ |
| Cortex | 477.70 ± 7.80 | 417.80 ± 7.54*** | 411.10 ± 5.06*** | 394.90 ± 7.06###, ^^^ | |
| Hippocampus | 443.80 ± 9.59 | 406.70 ± 12.83*** | 405.40 ± 4.84*** | 396.20 ± 7.84##, ^^ | |
| Cerebellum | 450.90 ± 7.14 | 414.10 ± 7.77*** | 417.50 ± 18.34*** | 397.10 ± 8.18#, ^^ | |
| GPx | Brain | 93.02 ± 5.07 | 77.80 ± 6.52*** | 125.20 ± 5.46*** | 100.30 ± 9.52###, ^^^ |
| Cortex | 96.76 ± 4.38 | 82.29 ± 6.52*** | 127.30 ± 5.93*** | 117.40 ± 4.97###, ^^ | |
| Hippocampus | 112.30 ± 5.40 | 96.67 ± 8.20*** | 122.90 ± 6.69* | 118.60 ± 7.68### | |
| Cerebellum | 123.30 ± 4.17 | 106.80 ± 6.95*** | 136.50 ± 6.11*** | 125.80 ± 4.73###, ^^^ | |
| SOD | Brain | 5.51 ± 0.30 | 4.95 ± 0.29*** | 5.45 ± 0.24 | 4.81 ± 0.19^^^ |
| Cortex | 11.87 ± 0.82 | 9.89 ± 0.93*** | 11.57 ± 1.00 | 9.15 ± 0.43^^^ | |
| Hippocampus | 25.22 ± 1.55 | 17.49 ± 2.47*** | 21.57 ± 0.92** | 18.59 ± 0.86^^^ | |
| Cerebellum | 9.39 ± 0.30 | 8.11 ± 0.79* | 8.53 ± 0.28 | 7.74 ± 0.41^^^ | |
| MDA | Brain | 19.11 ± 1.40 | 33.09 ± 1.41*** | 28.78 ± 1.05*** | 32.67 ± 0.70#, ^^^ |
| Cortex | 21.34 ± 1.01 | 35.58 ± 2.22*** | 33.95 ± 1.10*** | 71.28 ± 2.92###, ^^^ | |
| Hippocampus | 22.06 ± 0.54 | 29.65 ± 0.04*** | 29.50 ± 2.93*** | 39.11 ± 1.68###, ^^^ | |
| Cerebellum | 13.06 ± 0.56 | 25.40 ± 1.95***, ### | 21.46 ± 1.30***, ### | 31.05 ± 2.23*** | |
Rats were subjected to the CUMS procedure for 21 days. Data represent means ± SEM, n = 10 rats per group
*P < 0.05, **P < 0.01, ***P < 0.001 vs. unstressed saline-conditioned rats; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. unstressed nicotine-conditioned rats; ^P < 0.05, ^^P < 0.01, ^^^p < 0.001 vs. stressed saline-conditioned rats (Tukey’s test)