Table 7.
Influence of imipramine (15 mg/kg) on the parameters of oxidative stress (TAC, GPx, SOD, MDA) within the whole brain and its structures (cortex, hippocampus, and cerebellum) in stressed rats after 3 days of saline or nicotine (0.175 mg/kg, i.p.) conditioning
| Oxidative stress parameter | Conditioning | Saline | Nicotine | ||
|---|---|---|---|---|---|
| Treatment | Saline | Imipramine | Saline | Imipramine | |
| TAC | Brain | 428.50 ± 14.66 | 420.80 ± 6.56 | 386.40 ± 21.87*** | 402.60 ± 9.83 |
| Cortex | 411.10 ± 5.06 | 408.40 ± 6.44 | 382.40 ± 12.98*** | 394.90 ± 7.06#, ^ | |
| Hippocampus | 405.40 ± 4.84 | 406.40 ± 4.47 | 385.90 ± 11.36*** | 396.20 ± 7.84 | |
| Cerebellum | 417.50 ± 18.34 | 409.70 ± 8.67 | 395.80 ± 13.49* | 397.10 ± 8.18 | |
| GPx | Brain | 125.20 ± 5.46 | 118.90 ± 7.00 | 93.56 ± 6.84*** | 100.30 ± 9.52### |
| Cortex | 127.30 ± 5.93 | 121.60 ± 6.90 | 113.10 ± 9.05** | 117.40 ± 4.97 | |
| Hippocampus | 122.90 ± 6.69 | 139.20 ± 16.87* | 118.70 ± 3.48 | 118.60 ± 7.68## | |
| Cerebellum | 136.50 ± 6.11 | 129.30 ± 11.01 | 122.90 ± 6.78** | 125.80 ± 4.73 | |
| SOD | Brain | 5.45 ± 0.24 | 5.22 ± 0.19 | 4.80 ± 0.14*** | 4.81 ± 0.19## |
| Cortex | 11.57 ± 1.00 | 11.00 ± 0.82 | 8.75 ± 0.62*** | 9.15 ± 0.43### | |
| Hippocampus | 21.57 ± 0.92 | 19.80 ± 0.92 | 17.36 ± 2.42*** | 18.59 ± 0.86** | |
| Cerebellum | 8.53 ± 0.28 | 8.40 ± 0.20 | 7.21 ± 1.24** | 7.74 ± 0.41 | |
| MDA | Brain | 28.78 ± 1.05 | 28.54 ± 2.69 | 35.25 ± 1.20*** | 32.67 ± 0.70###, ^ |
| Cortex | 33.95 ± 1.10 | 39.32 ± 6.01 | 71.52 ± 5.08*** | 71.28 ± 2.92### | |
| Hippocampus | 29.50 ± 2.93 | 30.71 ± 1.09 | 39.69 ± 1.85*** | 39.11 ± 1.68### | |
| Cerebellum | 21.46 ± 1.30 | 22.74 ± 1.77 | 33.27 ± 2.07*** | 31.05 ± 2.23### | |
Rats were subjected to the CUMS procedure for 21 days. Data represent means ± SEM, n = 10 rats per group
*P < 0.05, **P < 0.01, ***P < 0.001 vs. stressed saline-treated, saline-conditioned rats; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. stressed imipramine-treated, saline-conditioned rats; ^P < 0.05, ^^P < 0.01, ^^^P < 0.001 vs. stressed saline-treated, nicotine-conditioned rats (Tukey’s test)