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. 2017 Apr 28;55(4):2897–2910. doi: 10.1007/s12035-017-0536-0

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Effects of monovalent and divalent cations (Na⁺, K⁺, Mg²⁺, Ca²⁺, and Zn²⁺) at concentrations of 500 μM and 5 mM on the binding of [³H]5-CT, [³H]SB-269970, and [³H]mesulergine to human recombinant 5-HT₇ receptors expressed in HEK293 cells. The binding of radioligands in the absence of the tested cations is referred to as basal specific binding and was set to 100% (dotted line)