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. 2018 Mar 7;8:19. doi: 10.1186/s13578-018-0216-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of KCNQ1OT1 in mMSCs stimulated with PMMA particles. a ALP activity in mMSCs transfected with pcDNA KCNQ1OT1 or pcDNA before the addition of PMMA particles. b The mRNA expression levels of Runx2, Osterix and OCN in mMSCs transfected with pcDNA KCNQ1OT1 or pcDNA before the addition of PMMA particles. c Osteoblastic differentiation in mMSCs transfected with pcDNA KCNQ1OT1 or pcDNA before the addition of PMMA particles assessed by ARS staining. d The relative expression levels of KCNQ1OT1 in mMSCs transfected with pcDNA KCNQ1OT1 or pcDNA. *P < 0.05 compared with pcDNA-transfected cells. *P < 0.05 compared with control group. ^&P < 0.05 compared with PMMA + pcDNA group