Fig. 1.

Flow chart outlining the study design and emphasis groups of the current vs. the companion paper [16]. A total of 69 subjects from the Karolinska COSMIC cohort, well-matched in terms of age, gender, and lung functions, were selected for iTRAQ proteomic investigations, including 18 healthy Never-smokers (9 male, 9 female), 25 Smokers with normal lung function (11 male, 14 female), 18 current smokers with COPD (10 male, 8 female), and 8 ex-smokers with COPD (3 male, 5 female). This manuscript focus on the alterations in proteomes and pathways related to COPD pathology (i.e., comparisons of Smoker vs. smokers with COPD, to some extent related to comparisons of healthy Never-smokers vs. ex-smokers with COPD (exCOPD)). The companion paper focuses on the effects of smoking prior to disease presentation, i.e., comparison of the Never-smoker vs Smoker groups [16]