Extended Data Figure 2.

Testing a minimal hairpin protospacer. a. Percent of arrays expanded with oligo-supplied spacers following electroporation of the sequences indicated below, aimed at testing PAM inclusion on both the top and bottom strands. Unfilled circles indicate individual biological replicates, bars are mean ±SEM. * indicates p<0.05. Oligos supplied at 3.125 μM each. b. Percent of arrays expanded with oligo-supplied spacers following electroporation of the sequences indicated to the left, right, and below aimed at finding a minimal functional hairpin protospacer. Unfilled circles indicate individual biological replicates, bars are mean ±SEM. Oligos supplied at 3.125 μM. c. Percent of arrays expanded following electroporation of different concentrations of the minimal hairpin oligo protospacer. Additional statistical details in Supplementary Table 2.