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. Author manuscript; available in PMC: 2018 Mar 8.
Published in final edited form as: Methods Mol Biol. 2018;1698:67–89. doi: 10.1007/978-1-4939-7428-3_4

Fig. 3.

Fig. 3

Phospho-flow cytometric analysis of cytokine signaling. Left: Flow cytometry plots of phospho-Akt after 10 min treatment with vehicle (black) or 10 ng/ml SCF (red) in control Ter119−/CD71high cells cultured in expansion medium for 24 h. Right: Phospho-Akt quantitation. MFI is expressed relative to unstimulated cells (mean ± SE, three biological replicates)